The Use of Chitosan and Whey Protein Isolate Edible Films Incorporated with Zataria multiflora Boiss. Essential Oil as an Active Packaging Ingredient Against Some Common Foodborne Bacteri

In this study, antimicrobial activity of chitosan and whey protein isolate edible films incorporated with different concentrations of zataria multiflora Boiss essential oil ;ZEO (1%, 2%, 3% and 4% v/v) and zataria multiflora Boiss essential oil nanoliposome (0, 1, 2, 3 and 4%) against  Listeria monocytogenes (ATCC 19118), Bacillus cereus (ATCC 11778), Staphylococcus aureus (ATCC 65138), Escherichia coli O157:H7(ATCC 35218) and vibrio parahaemolyticus (ATCC 43996) were evaluated using disc-diffusion test. The circular disks containing ZEO and NZEO were cut and placed on bacterial lawn. Plates were incubated for 24 h at 35° C. The inhibition zone diameter was measured by digital caliper. These inhibition zones were formed against Listeria monocytogenes (ATCC 19118), Bacillus cereus (ATCC 11778), Staphylococcus aureus (ATCC 65138), Escherichia coli O157:H7(ATCC 35218) and vibrio parahaemolyticus (ATCC 43996). The results of this study indicated that by increasing the concentration of ZEO in both whey protein isolate and chitosan films, a significant increase was observed in inhibition zones’ diameters (P